Next Generation Sequencing
The Biotechnology department has experience with amplicon library preparation, Illumina-based sequencing (Miseq), and bioinformatics using Qiime. Workflows have been developed and successfully performed for the following:
- Bacterial communities from various fish species
- Mucus, gills, skin, intestinal contents
- Water samples
- Microalgae, bacteria
- Bacteria samples from smolt stations and sea cages
- Tank growth, biofilters, sea cage shelters
Standard Illumina 16S rRNA amplicon workflow
Equipment and supplies
Firum´s laboratory space at iNOVA is fully equipped for molecular analysis with dedicated space for DNA/RNA work, a sterile LAF bench, and a separate post-PCR room. Specialized equipment for NGS workflows includes:
- NGS Library Preparation and Quality Control
- Tissue homogenizer (vortex adapter)
- Filtration system for water samples
- Magnetic racks for library clean-up
- Qubit (DNA/RNA concentration)
- Agilent Bioanalyzer (DNA/RNA quality control)
- ABI StepOnePlus (96 well, 0.2 ml format)
- Bio-rad standard thermocycler
- Next Generation sequencers
- Illumina Miseq
- Illumina Nextseq
Capillary Sequencing
An ABI 3130 Genetic Analyzer is also available for sequencing shorter DNA segments and validating qPCR primer specificity.
