DNA identification and abundance (qPCR)
The biotechnology department has established sample preparation and quantitative real-time PCR (sybrgreen and TaqMan probe) workflows for a variety of sample types and genetic markers including:
- Microalgae from water samples
- Nitrifying bacteria on biofilters
- Fin clips
- Salmon lice in stomach contents
Gene Expression analysis (qRT-PCR)
Workflows are currently being established in connection to the biobanking initiative so that we can measure changes in gene expression. The focus will be on sampling and preparation of high-quality RNA from mucosal tissues (gills, skin, mucus, and intestines) from lumpfish and Atlantic salmon and validating immune response biomarkers.
Equipment and supplies
Firum´s laboratory space at iNOVA is fully equipped for molecular analysis with dedicated space for DNA/RNA work, sterile LAF bench, and separate post-PCR room. Specialized equipment includes:
- Sample preparation
- Tissue homogenizers (vortex adapter)
- Filtration system for water samples
- Maxwell 16 (automated DNA extraction)
- Qubit (DNA/RNA concentration)
- Agilent Bioanalyzer (DNA/RNA quality control)
- Thermocyclers
- qPCR: ABI one-step plus (96 well, 0.2 ml format)
Standard PCR: G-Storm
